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.Development of a novel real-time RT-PCR assay for the rapid identification of Enterobacter hormaechei.
Enterobacter hormaechei is an opportunistic gram-negative human pathogen that commonly causes nosocomial infections. The clinical significance of E. hormaechei is increasing. The aim of this study was to develop a novel real-time RT-PCR assay to rapidly identify E. hormaechei. Primers and a probe targeting a 189-bp sequence in the tuf gene were used for the assay. The assay was carried out in triplicate on a Rotor-Gene 3000 real-time thermocycler using a conventional 25-μL reaction mixture containing 5-μL DNA template and 12.5-μL 2× SYBR Green PCR master mix (Roche Diagnostics, Basel, Switzerland). The assay was evaluated using 30 clinically significant E. hormaechei isolates and 72 non-E. hormaechei isolates. The detection limit of the assay was The present invention relates to a synchronous and or asynchronous network clock signal distribution system, and more particularly to a system for distributing a master clock signal to a number of local clocks, such as, for example, the local clocks on a number of separate computer chassis in ac619d1d87
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